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Part:BBa_I716302:Design

Designed by: vincent parker   Group: iGEM07_Berkeley_UC   (2007-06-27)

ots B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 832
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1919


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

PCR otsBF/otsBIR on otsB (485 bp, gp=A) PCR otsBIF/otsBR on otsB (381 bp, gp=B)

PCR otsBF/otsBR on A+B (832 bp, BglII/XhoI) Digest pBca9145-Bca1144 (BglII/XhoI, 2054+910, L) Product is pBca9145-BBa I716302

otsBF Forward BglII site anneals to otsB cgtaaAGATCTATGACAGAACCGTTAACCGA otsBIF Removes internal BglII site GCAACGTATTACTCAGATtTGGCCACAAATGGCG otsBIR Removes internal BglII site CGCCATTTGTGGCCAaATCTGAGTAATACGTTGC otsBR Reverse XhoI site anneals to otsB cgtatCTCGAGttaGGATCCTTAGATACTACGACTAAACG

References